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Senior Associate Scientist, Protein Biology & Crystallization

Company: Pfizer
Location: Boulder
Posted on: September 6, 2020

Job Description:

A career at Pfizer offers opportunity, ownership and impact. All over the world, Pfizer colleagues work together to positively impact health for everyone, everywhere. Our colleagues have opportunities to grow and develop a career that offers both individual and company success; be part of an ownership culture that values diversity and where all colleagues are energized and engaged; and the ability to impact the health and lives of millions of people. Pfizer, a global leader in the biopharmaceutical industry, is continuously seeking top talent who are inspired by our purpose to innovate to bring therapies to patients that significantly improve their lives.

Research unit overview

Pfizer- Boulder Research and Development, located in Boulder, Colorado USA, is a fully integrated drug discovery unit focusing on research and development of targeted small molecule therapeutic agents to treat patients afflicted with cancer.

Summary

We are seeking a protein biologist to join the Structural Biology group at Pfizer- Boulder Research and Development Unit. This individual will work closely with our protein biology experts to express, purify, characterize, and crystallize a variety of challenging proteins for use in both structural and mechanistic studies.

Responsibilities


  • Perform E. coli growth and recombinant protein expressions at liter-scale
  • Purify recombinant proteins from E. coli or eukaryotic host cells
  • Contribute to set up of protein crystallization trials and optimization
  • Assist with biochemical and/or biophysical characterization of proteins
  • Document experimental data
  • Contribute to written and oral presentations related to research findings

    Basic Qualifications

    • Minimum Bachelor of Science degree with 3+ years' post-graduate, or Master of Science with minimum 0-2 years' post-graduate, research experience.
    • Experience in recombinant protein expressions in E. coli
    • Experience in protein purification at mg-level yields with minimum supervision
    • Familiar with AKTA purifier system and standard analytical techniques associated with assessing protein purification process
    • Strong organizational, problem-solving, and written/oral communication skills

      Preferred Qualifications

      • Experience in protein crystallization resulted in diffracting crystals
      • Able to collect X-ray diffraction data from protein crystals
      • Experience in or familiar with biochemical or biophysical assays on protein functions

        Physical/Mental Requirements

        • Able to lift 50lb objects with dimensions 12 inches in height and 26 inches in width
        • Able to visualize microscopic objects under microscopes

          Non-Standard Work Schedule, Environment Requirements

          • Able to adhere to safe work practices and perform lab works with appropriate personal protection equipment such as but not limited to safety goggles, facial mask, gloves, lab coat/ protective gowning, and closed toe shoes
          • Able to work at flexible hours occasionally when performing time-sensitive experiments

            Other Job Details

            • Eligible for Employee Referral Bonus
            • #LI-PFE


              Sunshine Act

              Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.EEO & Employment Eligibility

              Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.Research and Development

Keywords: Pfizer, Boulder , Senior Associate Scientist, Protein Biology & Crystallization, Other , Boulder, Colorado

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