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Principal Scientist, Cryo-EM Crystallographer

Company: American Society for Biochemistry and Molecular Bi
Location: Boulder
Posted on: April 6, 2021

Job Description:

American Society for Biochemistry and Molecular Biology

  • Principal Scientist, Cryo-EM Crystallographer Principal Scientist, Cryo-EM Crystallographer ROLE SUMMARY
    We are seeking a structural biologist with strong expertise on single particle cryo-EM and X-ray crystallography to join the Structural Biology group at Pfizer-Boulder Research and Development Unit. This individual will establish and maintain a cryo-EM image processing pipeline for determining high resolution cryo-EM structures, and will also apply a broad range of structural biology tools to determine high resolution ligand-bound protein cryo-EM and X-ray crystal structures to support our Structure-based drug discovery programs. ROLE RESPONSIBILITIES:
    * Optimize Cryo-EM sample vitrification conditions and collect high magnification data.
    * Coordinate and manage cryo-EM data collection on multiple protein targets.
    * Design and conduct protein crystallization trials, crystal optimization, and X-ray data collection using both in-house X-ray generators and remoted data collection on synchrotrons.
    * Solve and refine crystallographic structures either by Molecular Replacement or de-novo.
    * Document experimental data.
    * Communicate research priority, progress, and results with project teams.
    * Contribute to written and oral presentations related to research findings.
    * Provides technical and scientific guidance to junior research associates within the team. BASIC QUALIFICATIONS
    * PhD in structural biology or a related field having 5+ years' experience with crystallography, and 2+ years of postdoctoral experience with cryo-EM that lead a proven track record of publications in reputable, peer-reviewed journals.
    * Experience with cryo-EM sample preparation protocols including negative staining and sample vitrification.
    * Experience with operating screening and high-resolution data-collection microscopes using programs such as EPU, SerialEM, and Leginon.
    * Expert in single particle cryo-EM image processing and 3D reconstruction using programs such as RELION, CryoSPARC, EMAN2.
    * Extensive experience in protein X-ray crystallography from crystallization, optimization, data collection and processing, to structure solution.
    * Experience with software for model building and refinement such as Phenix, CCP4-em, Chimera, Rosetta, Coot. PREFERRED QUALIFICATIONS
    * Experience with Linux OS and GPU-accelerated computational environment (Installation, configuration and maintenance).
    * Expert on data-processing and structure solution of small size protein and transient membrane protein complexes ( PHYSICAL/MENTAL REQUIREMENTS
    * Able to lift 50lb objects with dimensions 12 inches in height and 26 inches in width.
    * Able to visualize microscopic objects under microscopes. NON-STANDARD WORK SCHEDULE, ENVIRONMENT REQUIREMENTS
    * Able to adhere to safe work practices and perform lab works with appropriate personal protection equipment such as but not limited to safety goggles, facial mask, gloves, lab coat/ protective gowning, and closed toe shoes.
    * Able to work at flexible hours occasionally when performing time-sensitive experiments. OTHER JOB DETAILS
    * Eligible for Employee Referral Bonus
    * Eligible for Relocation #LI-PFE The annual base salary for this position ranges from $90,500 to $150,900. In addition, this position offers an annual bonus with a target of 15% of the base salary and eligibility to participate in our share based long term incentive program. Benefits offered include a retirement savings plan, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage in accordance with the terms and conditions of the applicable plans. Job Information
    • Job ID: 56255531
    • Position Title: Principal Scientist, Cryo-EM Crystallographer Please refer to the company's website or job descriptions to learn more about them. Scientist - Oligonucleotide Chemical Development Dicerna Dicerna Manufacturing Compliance Specialist AGC Biologics Boulder, CO, United States Quality Control Scientist, Equipment Specialist 11200 Rockville Pike, Suite 302Rockville, Maryland 20852(240) 283-6590

Keywords: American Society for Biochemistry and Molecular Bi, Boulder , Principal Scientist, Cryo-EM Crystallographer, Other , Boulder, Colorado

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